1. Possible sources of contamination of the nutrient medium in plant tissue culture are:
a.the medium itself
b.the culture vessel
c.the explant
d. the environment of the transfer area
e. the operator
choose the most appropriate answer from the options given below:
1. A, E only
2.B, C only
3. A,B,C,D only
4.A, B, C, D,E only
2. For fusion of protoplast this chemical is used as a fusogen
a. Potassium sulphate
b.Chloroform
c.Polyethylene Glycol (PEG)
d.Glycerol
3. Match List I with List II
List I List II
A. pBR322 I. EC Cockings
B. MS medium II. Kary Mullis
C. PCR reaction III. Boliver and Rodriquez
D. Protoplast Culture IV. Murashige and Skoog
Choose the correct answer from the options given below:
1. A-IV, B-III, C-II, D-I
2. A-I, B-II, C-IV, D-III
3. A-IV, B-I, C-III, D-II
4. A-III, B-IV, C-II, D-I
4. Cetyl trimethyl ammonium bromide (CTAB) method is generally used for
a. precipitating nucleic acids
b.precipitating protein
c.lipids
precipitating neutral polysaccharides
5. PCR involves the following steps. What is the correct sequence?
A. Visualisation of amplified product
B. PCR machine run
C. DNA isolation
D. Loading of PCR run
E. PCR reaction setup in tube
Choose the correct answer from the options given below:
a. A, B, C, D, E
b. C, E, D, B, A
c. B, A, D, C, E
d. E, B, D, C, A
Answers:
1. 3. A,B,C,D only
2. c.Polyethylene Glycol (PEG)
3. 4. A-III, B-IV, C-II, D-I
4.a. precipitating nucleic acids
5. b. C, E, D, B, A
🎯Practice Makes Perfect🎯
👉Practice More Questions on Economic Botany MCQ